isotope labeling การใช้

• A relative quantitation can be obtained with a variety of differential isotope labeling technologies.
• Support for the reaction mechanism is based on isotope labeling.
• Isotope labeling may also be used to identify the pathways used in phycotoxin biosynthesis.
• Radioactive isotope labeling experiments provide a powerful tool for determining the structure of organic molecules.
• This mechanism is supported by isotope labeling experiments.
• TAILS is also compatible with Stable isotope labeling by amino acids in cell culture ( SILAC ).
• Isotope labeling experiments and crossover experiments were essential to early efforts to understand the mechanism of aconitase action.
• With a 5-membered cycle in another round of isotope labeling studies in favor of the 4-membered cycle Chauvin mechanism:
• In protein recombinant, manipulated proteins are produced in large quantities and isotope labeling is a tool to test for relevant proteins.
• In the famous Meselson Stahl experiment of 1958 he and Frank Stahl demonstrated through nitrogen isotope labeling that DNA is replicated semi-conservatively.
• The figure demonstrates the use of stable isotope labeling to discover the carbon atom rearrangement through reactions using position specific labeled compounds.
• MFA combines the data harvested from isotope labeling with the stoichiometry of each reaction, matrix is constructed that contains the stoichiometry of the reactions.
• Unlike isotope labeling studies in organic and organometallic chemistry, which typically use deuterium when an isotope of hydrogen is desired, biochemical crossover experiments frequently employ tritium.
• In the first stage both oxygens are incorporated from the molecular oxygen while in the second step only one is incorporated as determined by isotope labeling studies.
• Many approaches can be taken for determining the identity of a terminator, including fluorescence labeling, mass labeling for mass spectrometry, measuring enzyme activity using a protein moiety, and isotope labeling.
• Metabolic flux analysis ( MFA ) using stable isotope labeling is an important tool for elucidating the flux of certain elements through the metabolic pathways and products, through each reaction.
• "' SILAC "'( "'Stable Isotope Labeling by / with Amino acids in Cell culture "') is a technique based on mass spectrometry that detects differences in protein abundance among samples using non-radioactive isotopic labeling.
• One important application is in " Stable isotope labeling with amino acids in cell culture " ( SILAC ) . 13 C-enriched compounds are used in medical diagnostic tests such as the urea breath test.
• Stable isotope labeling by amino acids in cell culture ( SILAC ) has emerged as an important method in secretomics  it helps to distinguish between secreted proteins and bovine serum contaminants in cell culture.
• Another application of isotope labeling would be in measuring DNA synthesis, that is cell proliferation " in vitro " . uses H 3-thymidine labeling to compare pattern of synthesis ( or sequence ) in cells.